A8601
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A8601
Sigma
G 418 disulfate salt
Biotechnology Performance Certified
| Synonym: | Antibiotic G418 |
| CAS Number: | 108321-42-2 |
| Linear Formula: | C20H40N4O10 · 2H2SO4 |
| Molecular Weight: | 692.71 |
| MDL number: | MFCD00058314 |
Description
| Biochem/physiol Actions | Mode of Action: Blocks polypeptide synthesis by inhibiting protein elongation. For use in the selection and maintenance of eukaryotic cells stably transfected with neomycin resistance genes. |
| Other Notes | Aminoglycoside antibiotic similar in structure to gentamicin, neomycin and kanamycin. |
| Legal Information | Geneticin is a registered trademark of Gibco BRL Life Technologies, Inc. |
Properties
| grade | Biotechnology Performance Certified |
| total impurities | Endotoxin, tested |
| suitability | cell culture tested |
| plant cell culture tested | |
| storage temp. | 2-8°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| Safety Statements | 22-24/25 |
| WGK Germany | 3 |
References
| reference | Kingston, R, Ausubel, F. et al., Stable transfer of genes into mammalian cells. Short Protocols in Molecular Biology 2nd ed., New York , (1992), 9.14 |
| Canaani, D., and Berg, P., Regulated expression of human interferon β1 gene after transduction in to cultured mouse and rabbit cells. Proc. Natl. Acad. Sci. U. S. A. 79, 5166-5170, (1982) | |
| Southern, P., and Berg, P., Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J. Mol. Biol. 1, 327-341, (1982) | |
| Halaban, R., and Alfano, F., Selective elimination of fibroblasts from cultures of normal human melanocytes. In Vitro Cell. Dev. Biol. 20, 447-450, (1984) | |
| Ursic, D., et al., A new antibiotic with known resistance factors, G418, inhibits plant cells. Biochem. Biophys. Res. Commun. 101, 1031-1037, (1981) | |
| Bar-Nun, S., et al., G-418, an elongation inhibitor of 80 S ribosomes. Biochim. Biophys. Acta 741, 123-127, (1983) |
